제품 > 항체 > 다중클론항체(pAb)

CCT8 Rabbit pAb (A4449)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CCT8 Rabbit pAb (A4449)

Western blot analysis of various lysates using CCT8 Rabbit pAb (A4449) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - CCT8 Rabbit pAb (A4449)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using CCT8 Rabbit pAb (A4449) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCCT8 Rabbit pAb
Catalog No.A4449
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 248-497 of human CCT8 (NP_001269837.1).
SequenceDMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND
Gene ID
Swiss Prot
SynonymsCctq; PRED71; D21S246; C21orf112; CCT8
Calculated MW60kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesB cells, LO2, 293T, HeLa, Mouse brain, Mouse liver, Mouse lung, Rat brain
Cellular locationCytoplasm, centrosome, cilium basal body, cytoskeleton, microtubule organizing center
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - CCT8 Rabbit pAb (A4449)}

    Western blot - CCT8 Rabbit pAb (A4449)

    Western blot analysis of various lysates using CCT8 Rabbit pAb (A4449) at 1:7000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - CCT8 Rabbit pAb (A4449)}

    Immunohistochemistry - CCT8 Rabbit pAb (A4449)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using CCT8 Rabbit pAb (A4449) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A4449? Please let us know so that we can cite the reference in this datasheet.