제품 > 항체 > 단일클론항체(mAb)

CD102 (ICAM2) Rabbit mAb (A23245)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using CD102 (ICAM2) Rabbit mAb (A23245) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - CD102 (ICAM2) Rabbit mAb (A23245)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD102 (ICAM2) Rabbit mAb (A23245) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human tonsil using CD102 (ICAM2) Rabbit mAb (A23245) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	CD102 (ICAM2) Rabbit mAb
Catalog No.	A23245
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59377

Background

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 25-223 of human CD102 (ICAM2) (NP_000864.2).
Sequence	KVFEVHVRPK KLAVEPKGSL EVNCSTTCNQ PEVGGLETSL DKILLDEQAQ WKHYLVSNIS HDTVLQCHFT CSGKQESMNS NVSVYQPPRQ VILTLQPTLV AVGKSFTIEC RVPTVEPLDS LTLFLFRGNE TLHYETFGKA APAPQEATAT FNSTADREDG HRNFSCLAVL DLMSRGGNIF HKHSAPKMLE IYEPVSDSQ
Gene ID
Swiss Prot
Synonyms	CD102; CD102 (ICAM2)
Calculated MW	30KDa
Observed MW	45kDa/55-80kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Jurkat, HUVEC
Cellular location	Membrane, Single-pass type I membrane protein