제품 > 항체 > 단일클론항체(mAb)

CD109 Rabbit mAb (A23787)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD109 Rabbit mAb (A23787)

Western blot analysis of various lysates, using CD109 Rabbit mAb (A23787) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):K-562
Exposure time: 60s.

Overview

Product nameCD109 Rabbit mAb
Catalog No.A23787
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61642
This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 223-462 of human CD109. (NP_598000.2).
SequenceEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKS
Gene ID
Swiss Prot
SynonymsCD109; CPAMD7; p180; r150; CD109 antigen
Calculated MW74kDa/152kDa/159kDa/161kDa
Observed MW190kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549
Cellular locationPlasma membrane, Cytosol

Documents

Certificate of Compliance

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ABclonal:Western blot - CD109 Rabbit mAb (A23787)}

Western blot - CD109 Rabbit mAb (A23787)

Western blot analysis of various lysates, using CD109 Rabbit mAb (A23787) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):K-562
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.