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CD115/CSF-1R Rabbit pAb (A3019)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD115/CSF-1R Rabbit pAb (A3019)

Western blot analysis of various lysates using CD115/CSF-1R Rabbit pAb (A3019) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of THP-1 cells using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of paraffin-embedded mouse bone marrow using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of THP-1 cells using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of paraffin-embedded mouse bone marrow using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD115/CSF-1R Rabbit pAb
Catalog No.A3019
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 873-972 of human CD115/CSF-1R (NP_005202.2).
SequenceYQMAQPAFAPKNIYSIMQACWALEPTHRPTFQQICSFLQEQAQEDRRERDYTNLPSSSRSGGSGSSSSELEEESSSEHLTCCEQGDIAQPLLQPNNYQFC
Gene ID
Swiss Prot
SynonymsFMS; CSFR; FIM2; GPSC; HDLS; C-FMS; CD115; HDLS1; CSF-1R; BANDDOS; M-CSF-R; CD115/CSF-1R
Calculated MW108kDa
Observed MW150-190kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, Mouse liver, Rat liver
Cellular locationCell membrane, Single-pass type I membrane protein
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD115/CSF-1R Rabbit pAb (A3019)}

Western blot - CD115/CSF-1R Rabbit pAb (A3019)

Western blot analysis of various lysates using CD115/CSF-1R Rabbit pAb (A3019) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)}

Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of THP-1 cells using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)}

Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of paraffin-embedded mouse bone marrow using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)}

Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of THP-1 cells using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)}

Immunofluorescence - CD115/CSF-1R Rabbit pAb (A3019)

Immunofluorescence analysis of paraffin-embedded mouse bone marrow using CD115/CSF-1R Rabbit pAb (A3019) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3019? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

재조합 단백질 (3)

Secondary Antibodies (25)