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CD155/PVR Rabbit mAb (A23084)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD155/PVR Rabbit mAb (A23084)

Western blot analysis of lysates from A-549 cells, using CD155/PVR Rabbit mAb (A23084) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using CD155/PVR Rabbit mAb (A23084) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CD155/PVR Rabbit mAb (A23084) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Overview

Product nameCD155/PVR Rabbit mAb
Catalog No.A23084
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59175
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-343 of human CD155/PVR (NP_006496.4).
SequenceWPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRN
Gene ID
Swiss Prot
SynonymsPVS; HVED; CD155; NECL5; TAGE4; Necl-5; CD155/PVR
Calculated MW45kDa
Observed MW70-80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesA549
Cellular locationCell membrane, Secreted.

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ABclonal:Western blot - CD155/PVR Rabbit mAb (A23084)}

Western blot - CD155/PVR Rabbit mAb (A23084)

Western blot analysis of lysates from A-549 cells, using CD155/PVR Rabbit mAb (A23084) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)}

Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using CD155/PVR Rabbit mAb (A23084) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)}

Immunohistochemistry - CD155/PVR Rabbit mAb (A23084)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CD155/PVR Rabbit mAb (A23084) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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