제품 > 항체 > 단일클론항체(mAb)

CD163 Rabbit mAb (A22619)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - CD163 Rabbit mAb (A22619)

Confocal imaging of paraffin-embedded Human liver tissue using CD163 Rabbit mAb (A22619, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CD163 Rabbit mAb (A22619)

Confocal imaging of paraffin-embedded Human spleen tissue using CD163 Rabbit mAb (A22619, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

Overview

Product nameCD163 Rabbit mAb
Catalog No.A22619
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58325
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 861-961 of human CD163 (NP_004235.4).
SequenceGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPKGPDTLWQCPSSPWEKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLD
Gene ID
Swiss Prot
SynonymsM130; MM130; SCARI1; CD163
Calculated MW125kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationCell membrane, Secreted
Customer validation

IF(Mus musculus)

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Immunofluorescence - CD163 Rabbit mAb (A22619)}

Immunofluorescence - CD163 Rabbit mAb (A22619)

Confocal imaging of paraffin-embedded Human liver tissue using CD163 Rabbit mAb (A22619, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - CD163 Rabbit mAb (A22619)}

Immunofluorescence - CD163 Rabbit mAb (A22619)

Confocal imaging of paraffin-embedded Human spleen tissue using CD163 Rabbit mAb (A22619, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A22619? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

ELISA 키트 (1)

재조합 단백질 (1)

제품 (2)

Secondary Antibodies (26)