제품 > 항체 > 다중클론항체(pAb)

CD163 Rabbit pAb (A8383)

Publications (21) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD163 Rabbit pAb (A8383)

Western blot analysis of lysates from 293T-CD163 cells, using CD163 Rabbit pAb (A8383) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human colon using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Rat liver using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD163 Rabbit pAb (A8383)

Immunofluorescence analysis of THP-1 cells using CD163 Rabbit pAb (A8383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD163 Rabbit pAb
Catalog No.A8383
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 750-1050 of human CD163 (NP_004235.4?).
SequenceLSDAHVVCRQLGCGEAINATGSAHFGEGTGPIWLDEMKCNGKESRIWQCHSHGWGQQNCRHKEDAGVICSEFMSLRLTSEASREACAGRLEVFYNGAWGTVGKSSMSETTVGVVCRQLGCADKGKINPASLDKAMSIPMWVDNVQCPKGPDTLWQCPSSPWEKRLASPSEETWITCDNKIRLQEGPTSCSGRVEIWHGGSWGTVCDDSWDLDDAQVVCQQLGCGPALKAFKEAEFGQGTGPIWLNEVKCKGNESSLWDCPARRWGHSECGHKEDAAVNCTDISVQKTPQKATTGRSSRQSS
Gene ID
Swiss Prot
SynonymsM130; MM130; SCARI1; CD163
Calculated MW125kDa
Observed MW160kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T-CD163
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.
Customer validation

IF(Mus musculus, Gallus gallus, Rattus norvegicus, Homo sapiens)

IHC(Homo sapiens, Rattus norvegicus, Mus musculus)

WB(Mus musculus, Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD163 Rabbit pAb (A8383)}

Western blot - CD163 Rabbit pAb (A8383)

Western blot analysis of lysates from 293T-CD163 cells, using CD163 Rabbit pAb (A8383) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human colon using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD163 Rabbit pAb (A8383)}

Immunohistochemistry - CD163 Rabbit pAb (A8383)

Immunohistochemistry analysis of paraffin-embedded Rat liver using CD163 Rabbit pAb (A8383) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD163 Rabbit pAb (A8383)}

Immunofluorescence - CD163 Rabbit pAb (A8383)

Immunofluorescence analysis of THP-1 cells using CD163 Rabbit pAb (A8383) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8383? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

ELISA 키트 (1)

재조합 단백질 (1)

제품 (2)

Secondary Antibodies (26)