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CD209 Rabbit mAb (A9649)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD209 Rabbit mAb (A9649)

Western blot analysis of various lysates using CD209 Rabbit mAb (A9649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CD209 Rabbit mAb (A9649)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD209 Rabbit mAb (A9649)

Immunofluorescence analysis of Raw264 7 cells using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD209 Rabbit mAb (A9649)

Immunofluorescence analysis of THP-1 cells using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD209 Rabbit mAb
Catalog No.A9649
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1679
This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including leprosy and tuberculosis mycobacteria, the Ebola, hepatitis C, HIV-1 and Dengue viruses, and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CLEC4M (Gene ID: 10332), also known as L-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression on the surface of dendritic cells. Polymorphisms in the neck region are associated with protection from HIV-1 infection, while single nucleotide polymorphisms in the promoter of this gene are associated with differing resistance and susceptibility to and severity of infectious disease, including rs4804803, which is associated with SARS severity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD209 (Q9NNX6).
SequenceMSDSKEPRLQQLGLLEEEQLRGLGFRQTRGYKSLAGCLGHGPLVLQLLSFTLLAGLLVQVSKVPSSISQEQSRQDAIYQNLTQLKAAVGELSEKSKLQEI
Gene ID
Swiss Prot
SynonymsCDSIGN; CLEC4L; DC-SIGN; DC-SIGN1; hDC-SIGN; CD209
Calculated MW46kDa
Observed MW45-55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, A-549, THP-1
Cellular locationCell membrane, Single-pass type II membrane protein, Secreted.
Customer validation

IHC(Mus musculus, Homo sapiens)

Documents

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ABclonal:Western blot - CD209 Rabbit mAb (A9649)}

Western blot - CD209 Rabbit mAb (A9649)

Western blot analysis of various lysates using CD209 Rabbit mAb (A9649) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD209 Rabbit mAb (A9649)}

Immunohistochemistry - CD209 Rabbit mAb (A9649)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD209 Rabbit mAb (A9649)}

Immunofluorescence - CD209 Rabbit mAb (A9649)

Immunofluorescence analysis of Raw264 7 cells using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD209 Rabbit mAb (A9649)}

Immunofluorescence - CD209 Rabbit mAb (A9649)

Immunofluorescence analysis of THP-1 cells using CD209 Rabbit mAb (A9649) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9649? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)