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CD22 Rabbit PolymAb® (A26417PM)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using CD22 Rabbit PolymAb® (A26417PM)at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): NIH/3T3
Exposure time: 45s.

ABclonal:Immunohistochemistry - CD22 Rabbit PolymAb® (A26417PM)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD22 Rabbit PolymAb® (A26417PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD22 Rabbit PolymAb® (A26417PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD22 Rabbit PolymAb® (A26417PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD22 Rabbit PolymAb® (A26417PM)

Confocal imaging of paraffin-embedded Mouse spleen tissue using CD22 Rabbit PolymAb® (A26417PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of Raji cells using CD22 Rabbit PolymAb® (A26417PM, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	CD22 Rabbit PolymAb®
Catalog No.	A26417PM
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Immunogen information

Immunogen	Recombinant protein of human/mouse CD22.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	SIGLEC2; SIGLEC-2
Calculated MW	95kDa/97kDa
Observed MW	130-140kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1500 - 1:6000 IHC-P 1:50 - 1:200 IF/ICC 1:1000 - 1:4000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Raji, Ramos, Mouse spleen, Mouse thymus
Cellular location	Cell membrane, Single-pass type I membrane protein, cell surface, cytoplasm, early endosome, external side of plasma membrane, extracellular exosome, plasma membrane, recycling endosome, Single-pass type I membrane protein.