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CD23 Rabbit mAb (A23376)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CD23 Rabbit mAb (A23376)

Western blot analysis of various lysates, using CD23 Rabbit mAb (A23376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameCD23 Rabbit mAb
Catalog No.A23376
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60396
Predicted to enable carbohydrate binding activity. Acts upstream of or within positive regulation of humoral immune response mediated by circulating immunoglobulin. Located in external side of plasma membrane. Orthologous to human FCER2 (Fc fragment of IgE receptor II).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-331 of mouse CD23 (NP_038545.1).
SequenceETEKNLKQLGDTAIQNVSHVTKDLQKFQSNQLAQKSQVVQMSQNLQELQAEQKQMKAQDSRLSQNLTGLQEDLRNAQSQNSKLSQNLNRLQDDLVNIKSLGLNEKRTASDSLEKLQEEVAKLWIEILISKGTACNICPKNWLHFQQKCYYFGKGSKQWIQARFACSDLQGRLVSIHSQKEQDFLMQHINKKDSWIGLQDLNMEGEFVWSDGSPVGYSNWNPGEPNNGGQGEDCVMMRGSGQWNDAFCRSYLDAWVCEQLATCEISAPLASVTPTRPTPKSEP
Gene ID
Swiss Prot
SynonymsCD23; Fce2; Fcer2; Ly-42
Calculated MW38kDa
Observed MW46kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesDaudi, Mouse spleen, Mouse testis
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CD23 Rabbit mAb (A23376)}

    Western blot - CD23 Rabbit mAb (A23376)

    Western blot analysis of various lysates, using CD23 Rabbit mAb (A23376) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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