제품 > 항체 > 다중클론항체(pAb)

CD275 Rabbit pAb (A23266)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from Mouse spleen, using CD275 Rabbit pAb (A23266) at 1:1500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	CD275 Rabbit pAb
Catalog No.	A23266
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable identical protein binding activity and signaling receptor binding activity. Involved in positive regulation of interleukin-4 production. Located in external side of plasma membrane. Is integral component of plasma membrane. Is expressed in several structures, including extraembryonic component; gonad; hemolymphoid system gland; liver; and stomach. Orthologous to human ICOSLG (inducible T cell costimulator ligand).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 47-270 of human CD275. (NP_056605.1).
Sequence	ETEVGAMVGSNVVLSCIDPHRRHFNLSGLYVYWQIENPEVSVTYYLPYKSPGINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRVFMNTATELVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLYWINTTDNSLIDTALQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENVALHQNITSISQAESFTGNNTKNP
Gene ID
Swiss Prot
Synonyms	B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; mKIAA0653; CD275
Calculated MW	36kDa
Observed MW	65-75kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse spleen
Cellular location