제품 > 항체 > 단일클론항체(mAb)

CD317/BST2 Rabbit mAb (A26077)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates using CD317/BST2 Rabbit mAb (A26077) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	CD317/BST2 Rabbit mAb
Catalog No.	A26077
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66244

Background

Predicted to enable metalloendopeptidase inhibitor activity and protein homodimerization activity. Involved in several processes, including negative regulation of intracellular transport of viral material; negative regulation of viral genome replication; and response to interferon-gamma. Located in cell surface. Is expressed in retina. Human ortholog(s) of this gene implicated in acquired immunodeficiency syndrome and endometrial cancer. Orthologous to human BST2 (bone marrow stromal cell antigen 2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 55-152 of mouse CD317/BST2 (NP_932763.1).
Sequence	SVACRDGLRAQAECRNTTHLLQRQLTRTQDSLLQAETQANSCNLTVVTLQESLEKKVSQALEQQARIKELENEVTKLNQELENLRIQKETSSTVQVNS
Gene ID
Swiss Prot
Synonyms	GREG; Bst-2; CD317; DAMP-1; 2310015I10Rik
Calculated MW	19kDa
Observed MW	28-40kDa/50-70kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse ovary, Mouse liver
Cellular location	Golgi apparatus, trans-Golgi network, Cell membrane, Late endosome, Membrane raft, Cytoplasm, Apical cell membrane