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CD3E+CD3G Rabbit mAb (A20213)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD3E+CD3G Rabbit mAb (A20213)

Western blot analysis of various lysates, using CD3E+CD3G Rabbit mAb (A20213) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)

Flow cytometric analysis of CD3E+CD3G Rabbit mAb (A20213) (1μg/mL) in Jurkats cells (orange) compare to rabbit IgG Isotype control (blue) and non-staining control (Red).

ABclonal:Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)

Human peripheral blood lymphocytes stained with CD3E+CD3G Rabbit mAb (A20213) (1μg/mL) (orange). Secondary antibody only staining (Blue) and unstained sample (red) were used as a control.

Overview

Product nameCD3E+CD3G Rabbit mAb
Catalog No.A20213
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5083-01
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-207(CD3E)& 1-182(CD3G) of human CD3 epsilon&CD3 gamma Heterodimer.
SequenceMQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI(CD3E)&MEQGKGLAVLILAIILLQGTLAQSIKGNHLVKVYDYQEDGSVLLTCDAEAKNITWFKDGKMIGFLTEDKKKWNLGSNAKDPRGMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLFAEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLRRN(CD3G)
Gene ID
Swiss Prot
SynonymsCD3E; IMD18; T3E; TCRE; CD3e molecule; CD3G; CD3-GAMMA; IMD17; T3G; CD3E+CD3G
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • FC 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Flow Cytometry    
Positive samplesJurkat, MOLT-4
Cellular locationCell membrane, Single-pass type I membrane protein, Membrane, Single-pass type I membrane protein
Customer validation

IF(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - CD3E+CD3G Rabbit mAb (A20213)}

    Western blot - CD3E+CD3G Rabbit mAb (A20213)

    Western blot analysis of various lysates, using CD3E+CD3G Rabbit mAb (A20213) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)}

    Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)

    Flow cytometric analysis of CD3E+CD3G Rabbit mAb (A20213) (1μg/mL) in Jurkats cells (orange) compare to rabbit IgG Isotype control (blue) and non-staining control (Red).
    ABclonal:Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)}

    Flow CytoMetry - CD3E+CD3G Rabbit mAb (A20213)

    Human peripheral blood lymphocytes stained with CD3E+CD3G Rabbit mAb (A20213) (1μg/mL) (orange). Secondary antibody only staining (Blue) and unstained sample (red) were used as a control.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20213? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)