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CD53 Rabbit pAb (A24527)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - CD53 Rabbit pAb (A24527)

Western blot analysis of Mouse spleen, using CD53 Rabbit pAb (A24527) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameCD53 Rabbit pAb
Catalog No.A24527
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable identical protein binding activity. Involved in positive regulation of myoblast fusion. Located in cell-cell junction and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human CD53 (CD53 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 107-181 of mouse CD53 (NP_031677.1).
SequenceEQKLNTLVAEGLNDSIQHYHSDNSTMKAWDFIQTQLQCCGVNGSSDWTSGPPSSCPSGADVQGCYNKAKSWFHSN
Gene ID
Swiss Prot
SynonymsOx-44; Tspan25
Calculated MW24kDa
Observed MW35-45kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse spleen
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CD53 Rabbit pAb (A24527)}

    Western blot - CD53 Rabbit pAb (A24527)

    Western blot analysis of Mouse spleen, using CD53 Rabbit pAb (A24527) at 1:800 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.