제품 > 항체 > 단일클론항체(mAb)

CD64 Rabbit mAb (A24022)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using CD64 Rabbit mAb (A24022) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product name	CD64 Rabbit mAb
Catalog No.	A24022
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53678

Background

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 16-288 of human CD64(NP_000557.1).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CD64; FCG1; FCRI; CD64A; FCGR1; IGFR1; FcgammaRI
Calculated MW	45-75kDa
Observed MW	45-75kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:20000 - 1:80000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1, U-937, HL-60
Cellular location	Cell membrane.
Customer validation	WB(Homo sapiens)