제품 > 항체 > 단일클론항체(mAb)

CD66a/CEACAM1 Rabbit mAb (A25475)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse liver using CD66a/CEACAM1a Rabbit mAb (A25475) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:30s.

ABclonal:Immunohistochemistry - CD66a/CEACAM1 Rabbit mAb (A25475)

Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using CD66a/CEACAM1a Rabbit mAb (A25475) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using CD66a/CEACAM1a Rabbit mAb (A25475) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD66a/CEACAM1 Rabbit mAb (A25475)

Confocal imaging of paraffin-embedded Mouse liver tissue using CD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded Mouse small intestine tissue using CD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Confocal imaging of paraffin-embedded Mouse spleen tissue using CD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Flow CytoMetry - CD66a/CEACAM1 Rabbit mAb (A25475)

Flow cytometry: 1X10^6 3T3-L1 cells (Low Expression, left) and C57BL/6 mouse Splenocytes (right) were surface-stained with CD66a/CEACAM1a Rabbit mAb (A25475, 2 μg/mL, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line), followed by PE conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or CD66a/CEACAM1a Rabbit mAb (A25475, 2 μg/mL, right).

Overview

Product name	CD66a/CEACAM1 Rabbit mAb
Catalog No.	A25475
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65927

Background

Enables several functions, including signaling receptor binding activity; virion binding activity; and virus receptor activity. Involved in several processes, including common myeloid progenitor cell proliferation; granulocyte colony-stimulating factor signaling pathway; and negative regulation of macromolecule metabolic process. Acts upstream of or within several processes, including modulation by host of viral process; regulation of T cell activation; and regulation of intracellular signal transduction. Located in cell projection membrane; external side of plasma membrane; and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and skeleton. Orthologous to several human genes including CEACAM1 (CEA cell adhesion molecule 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-428 of mouse CD66a/CEACAM1a (NP_001034274.1).
Sequence	EVTIEAVPPQVAEDNNVLLLVHNLPLALGAFAWYKGNTTAIDKEIARFVPNSNMNFTGQAYSGREIIYSNGSLLFQMITMKDMGVYTLDMTDENYRRTQATVRFHVHPILLKPNITSNNSNPVEGDDSVSLTCDSYTDPDNINYLWSRNGESLSEGDRLKLSEGNRTLTLLNVTRNDTGPYVCETRNPVSVNRSDPFSLNIIYGPDTPIISPSDIYLHPGSNLNLSCHAASNPPAQYFWLINEKPHASSQELFIPNITTNNSGTYTCFVNNSVTGLSRTTVKNITVLEPVTQPFLQVTNTTVKELDSVTLTCLSNDIGANIQWLFNSQSLQLTERMTLSQNNSILRIDPIKREDAGEYQCEISNPVSVRRSNSIKLDIIFDPTQGGLSDGAIAG
Gene ID
Swiss Prot
Synonyms	Bgp; Cc1; Hv2; Bgp1; Cea1; Cea7; Hv-2; MHVR; bb-1; C-CAM; CD66a; Cea-1; Cea-7; MHVR1; Mhv-1; mCEA1; mmCGM1; mmCGM2; mmCGM1a
Calculated MW	57kDa/50kDa/37kDa/30kDa
Observed MW	100-150kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:5000 - 1:20000 IHC-P 1:200 - 1:2000 IF/ICC 1:800 - 1:3200 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Flow Cytometry
Positive samples	Mouse liver
Cellular location	plasma membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

CD66a/CEACAM1 Rabbit mAb images

Western blot - CD66a/CEACAM1 Rabbit mAb (A25475)

Immunohistochemistry - CD66a/CEACAM1 Rabbit mAb (A25475)

Immunofluorescence - CD66a/CEACAM1 Rabbit mAb (A25475)

Flow CytoMetry - CD66a/CEACAM1 Rabbit mAb (A25475)

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or CD66a/CEACAM1a Rabbit mAb (A25475, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.