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CD68 Rabbit PolymAb® (A24386PM)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using CD68 Rabbit PolymAb® (A24386PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Jurkat
Exposure time: 10s.

ABclonal:Immunohistochemistry - CD68 Rabbit PolymAb® (A24386PM)

Immunohistochemistry analysis of paraffin-embedded Human colon using CD68 Rabbit PolymAb® (A24386PM) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using CD68 Rabbit PolymAb® (A24386PM) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung using CD68 Rabbit PolymAb® (A24386PM) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD68 Rabbit PolymAb® (A24386PM) at dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD68 Rabbit PolymAb® (A24386PM)

Immunofluorescence analysis of RAW264.7 cells using CD68 Rabbit PolymAb® (A24386PM) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	CD68 Rabbit PolymAb®
Catalog No.	A24386PM
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in several processes, including cellular response to lipopolysaccharide; cellular response to oxidised low-density lipoprotein particle stimulus; and negative regulation of dendritic cell antigen processing and presentation. Acts upstream of or within aging and cellular response to organic substance. Located in lysosome and plasma membrane. Is expressed in several structures, including central nervous system; embryo mesenchyme; heart blood vessel; liver; and spleen. Orthologous to human CD68 (CD68 molecule).

Immunogen information

Immunogen	Recombinant protein of human/mouse CD68.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	Lamp4; gp110; Scard1
Calculated MW	70-130kDa
Observed MW	70-130kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:5000 - 1:14000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	U-937
Cellular location