제품 > 항체 > 다중클론항체(pAb)

CD74 Rabbit pAb (A24487)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - CD74 Rabbit pAb (A24487)

Western blot analysis of Mouse thymus, using CD74 Rabbit pAb (A24487) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCD74 Rabbit pAb
Catalog No.A24487
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables MHC class II protein binding activity; cytokine receptor activity; and nitric-oxide synthase binding activity. Contributes to cytokine binding activity. Involved in several processes, including macrophage migration inhibitory factor signaling pathway; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of T cell differentiation; and thymic T cell selection. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and multivesicular body. Part of MHC class II protein complex; NOS2-CD74 complex; and macrophage migration inhibitory factor receptor complex. Is expressed in lower jaw; lower jaw incisor; lower jaw molar; and thymus primordium. Orthologous to human CD74 (CD74 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 56-215 of mouse CD74 (NP_034675.1).
SequenceQQQGRLDKLTITSQNLQLESLRMKLPKSAKPVSQMRMATPLLMRPMSMDNMLLGPVKNVTKYGNMTQDHVMHLLTRSGPLEYPQLKGTFPENLKHLKNSMDGVNWKIFESWMKQWLLFEMSKNSLEEKKPTEAPPKEPLDMEDLSSGLGVTRQELGQVTL
Gene ID
Swiss Prot
SynonymsIi; CLIP; DHLAG; HLADG; Ia-GAMMA
Calculated MW24kDa
Observed MW34kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse thymus
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CD74 Rabbit pAb (A24487)}

    Western blot - CD74 Rabbit pAb (A24487)

    Western blot analysis of Mouse thymus, using CD74 Rabbit pAb (A24487) at 1:900 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.