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CD79B Rabbit mAb (A4362)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD79B Rabbit mAb (A4362)

Western blot analysis of various lysates, using CD79B Rabbit mAb (A4362) at 1:3600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CD79B Rabbit mAb (A4362)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD79B Rabbit mAb (A4362) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD79B Rabbit mAb (A4362)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD79B Rabbit mAb (A4362) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded Human spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded mouse spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded rat spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCD79B Rabbit mAb
Catalog No.A4362
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52688
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD79B (P40259).
SequenceMARLALSPVPSHWMVALLLLLSAEPVPAARSEDRYRNPKGSACSRIWQSPRFIARKRGFTVKMHCYMNSASGNVSWLWKQEMDENPQQLKLEKGRMEESQ
Gene ID
Swiss Prot
SynonymsB29; IGB; AGM6; Igbeta; CD79B
Calculated MW26kDa
Observed MW30-50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesDaudi, Raji, Rat spleen
Cellular locationexternal side of plasma membrane, extracellular exosome, plasma membrane.

Documents

Certificate of Compliance

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ABclonal:Western blot - CD79B Rabbit mAb (A4362)}

Western blot - CD79B Rabbit mAb (A4362)

Western blot analysis of various lysates, using CD79B Rabbit mAb (A4362) at 1:3600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CD79B Rabbit mAb (A4362)}

Immunohistochemistry - CD79B Rabbit mAb (A4362)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD79B Rabbit mAb (A4362) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD79B Rabbit mAb (A4362)}

Immunohistochemistry - CD79B Rabbit mAb (A4362)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD79B Rabbit mAb (A4362) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)}

Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded Human spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)}

Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded mouse spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - CD79B Rabbit mAb (A4362)}

Immunofluorescence - CD79B Rabbit mAb (A4362)

Confocal imaging of paraffin-embedded rat spleen using CD79B Rabbit mAb (A4362, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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