제품 > 항체 > 다중클론항체(pAb)

CD81 Rabbit pAb (A24484)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CD81 Rabbit pAb (A24484)

Western blot analysis of Mouse brain, using CD81 Rabbit pAb (A24484) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - CD81 Rabbit pAb (A24484)

Western blot analysis of Rat brain, using CD81 Rabbit pAb (A24484) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameCD81 Rabbit pAb
Catalog No.A24484
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable cholesterol binding activity; signaling receptor binding activity; and virus receptor activity. Involved in several processes, including cellular response to low-density lipoprotein particle stimulus; positive regulation of immune response; and syncytium formation by plasma membrane fusion. Acts upstream of or within regulation of cell motility. Located in membrane. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in common variable immunodeficiency. Orthologous to human CD81 (CD81 molecule).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 106-211 of mouse CD81(NP_598416.1).
SequenceVAAGIWGFVNKDQIAKDVKQFYDQALQQAVMDDDANNAKAVVKTFHETLNCCGSNALTTLTTTILRNSLCPSGGNILTPLLQQDCHQKIDELFSGKLYLIGIAAIV
Gene ID
Swiss Prot
SynonymsTapa1; Tapa-1; Tspan28
Calculated MW25kDa
Observed MW22kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CD81 Rabbit pAb (A24484)}

    Western blot - CD81 Rabbit pAb (A24484)

    Western blot analysis of Mouse brain, using CD81 Rabbit pAb (A24484) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Western blot - CD81 Rabbit pAb (A24484)}

    Western blot - CD81 Rabbit pAb (A24484)

    Western blot analysis of Rat brain, using CD81 Rabbit pAb (A24484) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.