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CD83 Rabbit mAb (A4234)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD83 Rabbit mAb (A4234)

Western blot analysis of various lysates using CD83 Rabbit mAb (A4234) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - CD83 Rabbit mAb (A4234)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD83 Rabbit mAb (A4234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CD83 Rabbit mAb (A4234)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD83 Rabbit mAb (A4234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD83 Rabbit mAb
Catalog No.A4234
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0935
The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD83 (Q01151).
SequenceMSRGLQLLLLSCAYSLAPATPEVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRNTTSC
Gene ID
Swiss Prot
SynonymsBL11; HB15; CD83
Calculated MW23kDa
Observed MW25-80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Raji, U-937, Mouse liver, Mouse brain, Rat brain
Cellular locationMembrane, .
Customer validation

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD83 Rabbit mAb (A4234)}

Western blot - CD83 Rabbit mAb (A4234)

Western blot analysis of various lysates using CD83 Rabbit mAb (A4234) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - CD83 Rabbit mAb (A4234)}

Immunofluorescence - CD83 Rabbit mAb (A4234)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD83 Rabbit mAb (A4234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CD83 Rabbit mAb (A4234)}

Immunofluorescence - CD83 Rabbit mAb (A4234)

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD83 Rabbit mAb (A4234) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4234? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)