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CDC40 Rabbit mAb (A19794)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CDC40 Rabbit mAb (A19794)

Western blot analysis of various lysates using CDC40 Rabbit mAb (A19794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat testis tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human breast cancer tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human spleen tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse brain tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse testis tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat lung tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat colon tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse liver tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human small intestine tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat kidney tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse colon tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameCDC40 Rabbit mAb
Catalog No.A19794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2321
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDC40 (O60508).
SequenceMSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFR
Gene ID
Swiss Prot
SynonymsEHB3; PCH15; PRP17; PRPF17; CDC40
Calculated MW66kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HCT116, HepG2, Mouse lung, Mouse brain, Mouse spleen, Rat lung
Cellular locationnuclear speck, nucleoplasm

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CDC40 Rabbit mAb (A19794)}

Western blot - CDC40 Rabbit mAb (A19794)

Western blot analysis of various lysates using CDC40 Rabbit mAb (A19794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat testis tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human breast cancer tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human spleen tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse brain tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse testis tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat lung tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat colon tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse liver tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded human small intestine tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded rat kidney tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDC40 Rabbit mAb (A19794)}

Immunohistochemistry - CDC40 Rabbit mAb (A19794)

Immunohistochemistry analysis of CDC40 in paraffin-embedded mouse colon tissue using CDC40 Rabbit mAb (A19794) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)