제품 > 항체 > 다중클론항체(pAb)

CDCA7L Rabbit pAb (A15472)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CDCA7L Rabbit pAb (A15472)

Western blot analysis of various lysates using CDCA7L Rabbit pAb (A15472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCDCA7L Rabbit pAb
Catalog No.A15472
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Acts upstream of or within positive regulation of cell population proliferation. Located in cytosol; fibrillar center; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDCA7L (NP_061189.2).
SequenceMELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRMNPTRSARPPEKFALENFTVSAAKFAEEFYSF
Gene ID
Swiss Prot
SynonymsR1; JPO2; RAM2; CDCA7L
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, Jurkat, Mouse spleen, Mouse brain, Rat brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - CDCA7L Rabbit pAb (A15472)}

Western blot - CDCA7L Rabbit pAb (A15472)

Western blot analysis of various lysates using CDCA7L Rabbit pAb (A15472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.