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CDK12 Rabbit mAb (A22474)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Human heart using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Human spleen using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Rat testis using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCDK12 Rabbit mAb
Catalog No.A22474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53277
Enables RNA polymerase II CTD heptapeptide repeat kinase activity and cyclin binding activity. Involved in phosphorylation of RNA polymerase II C-terminal domain; protein autophosphorylation; and regulation of MAP kinase activity. Located in nuclear speck. Part of cyclin K-CDK12 complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 420-516 of human CDK12 (NP_057591.2).
SequenceSKGSPVFLPRKENSSVEAKDSGLESKKLPRSVKLEKSAPDTELVNVTHLNTEVKNSSDTGKVKLDENSEKHLVKDLKAQGTRDSKPIALKEEIVTPK
Gene ID
Swiss Prot
SynonymsCRK7; CRKR; CRKRS; CDK12
Calculated MW164kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    
Positive samples
Cellular locationNucleus, Nucleus speckle.

Documents

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ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)}

Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Human heart using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)}

Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Human spleen using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)}

Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CDK12 Rabbit mAb (A22474)}

Immunohistochemistry - CDK12 Rabbit mAb (A22474)

Immunohistochemistry analysis of paraffin-embedded Rat testis using CDK12 Rabbit mAb (A22474) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (25)