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CDKAL1 Rabbit pAb (A5771)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CDKAL1 Rabbit pAb (A5771)

Western blot analysis of extracts of various cell lines, using CDKAL1 antibody (A5771) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CDKAL1 Rabbit pAb (A5771)

Immunofluorescence analysis of U2OS cells using CDKAL1 antibody (A5771). Blue: DAPI for nuclear staining.

Overview

Product nameCDKAL1 Rabbit pAb
Catalog No.A5771
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CDKAL1 (NP_060244.2).
SequenceMPSASCDTLLDDIEDIVSQEDSKPQDRHFVRKDVVPKVRRRNTQKYLQEEENSPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKIVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNL
Gene ID
Swiss Prot
SynonymsCDKAL1
Calculated MW65kDa
Observed MW55-65kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHL-60, HeLa, BT-474, 293T, HepG2, Mouse kidney, Mouse heart, Mouse spleen, Mouse lung
Cellular locationEndoplasmic reticulum membrane, Single-pass membrane protein

Documents

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    ABclonal:Western blot - CDKAL1 Rabbit pAb (A5771)}

    Western blot - CDKAL1 Rabbit pAb (A5771)

    Western blot analysis of extracts of various cell lines, using CDKAL1 antibody (A5771) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CDKAL1 Rabbit pAb (A5771)}

    Immunofluorescence - CDKAL1 Rabbit pAb (A5771)

    Immunofluorescence analysis of U2OS cells using CDKAL1 antibody (A5771). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.