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CELA2A Rabbit pAb (A17200)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CELA2A Rabbit pAb (A17200)

Western blot analysis of lysates from Mouse lung, using CELA2A Rabbit pAb (A17200) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CELA2A Rabbit pAb (A17200)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using CELA2A Rabbit pAb (A17200) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCELA2A Rabbit pAb
Catalog No.A17200
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-150 of human CELA2A (NP_254275.1).
SequenceTYRVGLGRHNLYVAESGSLAVSVSKIVVHKDWNSNQISKGNDIALLKLANPVSLTDKIQLACLPPAGTILP
Gene ID
Swiss Prot
SynonymsPE-1; AOMS4; ELA2A; CELA2A
Calculated MW29kDa
Observed MW29kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse lung
Cellular locationcytosol, extracellular region, extracellular space, keratohyalin granule

Documents

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    ABclonal:Western blot - CELA2A Rabbit pAb (A17200)}

    Western blot - CELA2A Rabbit pAb (A17200)

    Western blot analysis of lysates from Mouse lung, using CELA2A Rabbit pAb (A17200) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CELA2A Rabbit pAb (A17200)}

    Immunofluorescence - CELA2A Rabbit pAb (A17200)

    Immunofluorescence analysis of paraffin-embedded mouse pancreas using CELA2A Rabbit pAb (A17200) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.