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CEMIP Rabbit pAb (A8587)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CEMIP Rabbit pAb (A8587)

Western blot analysis of various lysates using CEMIP Rabbit pAb (A8587) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product nameCEMIP Rabbit pAb
Catalog No.A8587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables several functions, including clathrin heavy chain binding activity; hyaluronic acid binding activity; and hyalurononglucosaminidase activity. Involved in several processes, including hyaluronan catabolic process; positive regulation of protein phosphorylation; and positive regulation of transport. Located in clathrin-coated endocytic vesicle; endoplasmic reticulum; and plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 180-420 of human CEMIP (NP_001280227.1).
SequenceFERSWGHRGVIVHVIDPKSGTVIHSDRFDTYRSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSSSVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAHPGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFACYDRGRACRSYRVRFLCGKPVRPKLTVTIDTNVN
Gene ID
Swiss Prot
SynonymsCCSP1; HYBID; TMEM2L; KIAA1199; CEMIP
Calculated MW153kDa
Observed MW180kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431, U-87MG, Mouse brain
Cellular locationCell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus, Secreted, clathrin-coated pit
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Homo sapiens)

    ABclonal:Western blot - CEMIP Rabbit pAb (A8587)}

    Western blot - CEMIP Rabbit pAb (A8587)

    Western blot analysis of various lysates using CEMIP Rabbit pAb (A8587) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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