Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat
Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (A3975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | CENPC Rabbit mAb |
|---|---|
| Catalog No. | A3975 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC2097 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 380-520 of human CENPC (Q03188). |
|---|---|
| Sequence | VLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | MIF2; hcp-4; CENP-C; CENPC1 |
| Calculated MW | 107kDa |
| Observed MW | 107kDa |
| Reactivity | Human, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | HeLa, Jurkat |
| Cellular location | cytosol, nuclear body, nucleoplasm |
| Customer validation | IF(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A3975? Please let us know so that we can cite the reference in this datasheet.
