제품 > 항체 > 다중클론항체(pAb)

CEP55 Rabbit pAb (A8229)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CEP55 Rabbit pAb (A8229)

Western blot analysis of extracts of THP-1 cells, using CEP55 antibody (A8229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - CEP55 Rabbit pAb (A8229)

Immunofluorescence analysis of L929 cells using CEP55 antibody (A8229) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCEP55 Rabbit pAb
Catalog No.A8229
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Involved in cranial skeletal system development; establishment of protein localization; and midbody abscission. Acts upstream of or within mitotic cytokinesis. Located in Flemming body; centriolar satellite; and plasma membrane. Implicated in multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CEP55 (NP_060601.3).
SequenceMSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTALLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSF
Gene ID
Swiss Prot
SynonymsCT111; MARCH; URCC6; C10orf3
Calculated MW54kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesTE-1
Cellular locationCleavage furrow, Cytoplasm, Midbody, centriole, centrosome, cytoskeleton, microtubule organizing center

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ABclonal:Western blot - CEP55 Rabbit pAb (A8229)}

Western blot - CEP55 Rabbit pAb (A8229)

Western blot analysis of extracts of THP-1 cells, using CEP55 antibody (A8229) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - CEP55 Rabbit pAb (A8229)}

Immunofluorescence - CEP55 Rabbit pAb (A8229)

Immunofluorescence analysis of L929 cells using CEP55 antibody (A8229) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.