제품 > 항체 > 다중클론항체(pAb)

CEP57L1 Rabbit pAb (A8290)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CEP57L1 Rabbit pAb (A8290)

Western blot analysis of extracts of various cell lines, using CEP57L1 antibody (A8290) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCEP57L1 Rabbit pAb
Catalog No.A8290
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Predicted to be located in cytoplasm and microtubule. Predicted to be active in centrosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CEP57L1 (NP_776191.1).
SequenceMDSELMHSIVGSYHKPPERVFVPSFTQNEPSQNCHPANLEVTSPKILHSPNSQALILALKTLQEKIHRLELERTQAEDNLNILSREAAQYKKALENETNERNLAHQELIKQKKDISIQLSSAQSRCTLLEKQLEYTKRMVLNVEREKNMILEQQAQLQREKEQDQMK
Gene ID
Swiss Prot
Synonymscep57R; C6orf182; bA487F23.2
Calculated MW54kDa
Observed MW60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples5637, BT-474, 22Rv1
Cellular locationCytoplasm, centrosome, cytoskeleton, microtubule organizing center

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CEP57L1 Rabbit pAb (A8290)}

Western blot - CEP57L1 Rabbit pAb (A8290)

Western blot analysis of extracts of various cell lines, using CEP57L1 antibody (A8290) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.