제품 > 항체 > 다중클론항체(pAb)

CFHR3 Rabbit pAb (A7775)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CFHR3 Rabbit pAb (A7775)

Western blot analysis of Rat liver, using CFHR3 Rabbit pAb (A7775) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CFHR3 Rabbit pAb (A7775)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse liver cells using CFHR3 Rabbit pAb (A7775) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CFHR3 Rabbit pAb (A7775)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of rat liver cells using CFHR3 Rabbit pAb (A7775) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCFHR3 Rabbit pAb
Catalog No.A7775
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human CFHR3 (NP_066303.2).
SequenceAVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQ
Gene ID
Swiss Prot
SynonymsFHR3; HLF4; CFHL3; FHR-3; DOWN16
Calculated MW37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat liver
Cellular locationSecreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CFHR3 Rabbit pAb (A7775)}

    Western blot - CFHR3 Rabbit pAb (A7775)

    Western blot analysis of Rat liver, using CFHR3 Rabbit pAb (A7775) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - CFHR3 Rabbit pAb (A7775)}

    Immunofluorescence - CFHR3 Rabbit pAb (A7775)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of mouse liver cells using CFHR3 Rabbit pAb (A7775) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CFHR3 Rabbit pAb (A7775)}

    Immunofluorescence - CFHR3 Rabbit pAb (A7775)

    Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of rat liver cells using CFHR3 Rabbit pAb (A7775) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.