제품 > 항체 > 다중클론항체(pAb)

CHAMP1 Rabbit pAb (A11690)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CHAMP1 Rabbit pAb (A11690)

Western blot analysis of various lysates using CHAMP1 Rabbit pAb (A11690) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - CHAMP1 Rabbit pAb (A11690)

Immunofluorescence analysis of Hela cells using CHAMP1 Rabbit pAb (A11690) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCHAMP1 Rabbit pAb
Catalog No.A11690
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a zinc finger protein that functions as a regulator of chromosome segregation in mitosis. The encoded protein is required for correct alignment of chromosomes on the metaphase plate, and plays a role in maintaining the attachment of sister kinetochores to microtubules from opposite spindle poles. Mutations in this gene are associated with an autosomal dominant form of intellectual disability.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 563-812 of human CHAMP1 (NP_115812.1).
SequenceLPKSALFSESQKAVELGDELQIDAIDDQKCDILVQEELLASPKKLLEDTLFPSSKKLKKDNQESSDAELSSSEYIKTDLDAMDIKGQESSSDQEQVDVESIDFSKENKMDMTSPEQSRNVLQFTEEKEAFISEEEIAKYMKRGKGKYYCKICCCRAMKKGAVLHHLVNKHNVHSPYKCTICGKAFLLESLLKNHVAAHGQSLLKCPRCNFESNFPRGFKKHLTHCQSRHNEEANKKLMEALEPPLEEQQI
Gene ID
Swiss Prot
SynonymsCAMP; CHAMP; MRD40; ZNF828; C13orf8; NEDHILD; CHAMP1
Calculated MW89kDa
Observed MW130kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, A-431, Jurkat
Cellular locationChromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CHAMP1 Rabbit pAb (A11690)}

    Western blot - CHAMP1 Rabbit pAb (A11690)

    Western blot analysis of various lysates using CHAMP1 Rabbit pAb (A11690) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - CHAMP1 Rabbit pAb (A11690)}

    Immunofluorescence - CHAMP1 Rabbit pAb (A11690)

    Immunofluorescence analysis of Hela cells using CHAMP1 Rabbit pAb (A11690) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.