제품 > 항체 > 다중클론항체(pAb)

CHCHD1 Rabbit pAb (A15941)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CHCHD1 Rabbit pAb (A15941)

Western blot analysis of various lysates using CHCHD1 Rabbit pAb (A15941) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameCHCHD1 Rabbit pAb
Catalog No.A15941
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Predicted to be involved in mitochondrial translation. Located in cytosol; mitochondrion; and nuclear lumen.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human CHCHD1 (NP_976043.1).
SequenceMATPSLRGRLARFGNPRKPVLKPNKPLILANRVGERRREKGEATCITEMSVMMACWKQNEFRDDACRKEIQGFLDCAARAQEARKMRSIQETLGESGSLLPNKLNKLLQRFPNKPYLS
Gene ID
Swiss Prot
SynonymsC2360; MRP-S37; C10orf34; CHCHD1
Calculated MW13kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Mouse liver
Cellular locationMitochondrion, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - CHCHD1 Rabbit pAb (A15941)}

    Western blot - CHCHD1 Rabbit pAb (A15941)

    Western blot analysis of various lysates using CHCHD1 Rabbit pAb (A15941) at 1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.