제품 > 항체 > 다중클론항체(pAb)

CHI3L2 Rabbit pAb (A7654)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CHI3L2 Rabbit pAb (A7654)

Western blot analysis of Jurkat, using CHI3L2 Rabbit pAb (A7654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CHI3L2 Rabbit pAb (A7654)

Immunohistochemistry analysis of paraffin-embedded human liver using CHI3L2 Rabbit pAb (A7654) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCHI3L2 Rabbit pAb
Catalog No.A7654
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-390 of human CHI3L2 (NP_003991.2).
SequenceYKLVCYFTNWSQDRQEPGKFTPENIDPFLCSHLIYSFASIENNKVIIKDKSEVMLYQTINSLKTKNPKLKILLSIGGYLFGSKGFHPMVDSSTSRLEFINSIILFLRNHNFDGLDVSWIYPDQKENTHFTVLIHELAEAFQKDFTKSTKERLLLTAGVSAGRQMIDNSYQVEKLAKDLDFINLLSFDFHGSWEKPLITGHNSPLSKGWQDRGPSSYYNVEYAVGYWIHKGMPSEKVVMGIPTYGHSFTLASAETTVGAPASGPGAAGPITESSGFLAYYEICQFLKGAKITRLQDQQVPYAVKGNQWVGYDDVKSMETKVQFLKNLNLGGAMIWSIDMDDFTGKSCNQGPYPLVQAVKRSLGSL
Gene ID
Swiss Prot
SynonymsCHIL2; YKL39; YKL-39
Calculated MW44kDa
Observed MW40kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat
Cellular locationSecreted

Documents

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ABclonal:Western blot - CHI3L2 Rabbit pAb (A7654)}

Western blot - CHI3L2 Rabbit pAb (A7654)

Western blot analysis of Jurkat, using CHI3L2 Rabbit pAb (A7654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - CHI3L2 Rabbit pAb (A7654)}

Immunohistochemistry - CHI3L2 Rabbit pAb (A7654)

Immunohistochemistry analysis of paraffin-embedded human liver using CHI3L2 Rabbit pAb (A7654) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.