제품 > 항체 > 다중클론항체(pAb)

CHML Rabbit pAb (A19306)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - CHML Rabbit pAb (A19306)

Western blot analysis of lysates from Rat brain using CHML Rabbit pAb (A19306) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CHML Rabbit pAb (A19306)

Immunofluorescence analysis of L929 cells using CHML Rabbit pAb (A19306) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCHML Rabbit pAb
Catalog No.A19306
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of the CHML gene supports geranylgeranylation of most Rab proteins and may substitute for REP-1 in tissues other than retina. CHML is localized close to the gene for Usher syndrome type II.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHML (NP_001812.2).
SequenceMADNLPTEFDVVIIGTGLPESILAAACSRSGQRVLHIDSRSYYGGNWASFSFSGLLSWLKEYQQNNDIGEESTVVWQDLIHETEEAITLRKKDETIQHTE
Gene ID
Swiss Prot
SynonymsREP2; CHML
Calculated MW74kDa
Observed MW74kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationcytoplasm, cytosol, nucleoplasm, nucleus, Rab-protein geranylgeranyltransferase complex

Documents

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    ABclonal:Western blot - CHML Rabbit pAb (A19306)}

    Western blot - CHML Rabbit pAb (A19306)

    Western blot analysis of lysates from Rat brain using CHML Rabbit pAb (A19306) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CHML Rabbit pAb (A19306)}

    Immunofluorescence - CHML Rabbit pAb (A19306)

    Immunofluorescence analysis of L929 cells using CHML Rabbit pAb (A19306) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.