제품 > 항체 > 다중클론항체(pAb)

CHP1 Rabbit pAb (A15791)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CHP1 Rabbit pAb (A15791)

Western blot analysis of various lysates using CHP1 Rabbit pAb (A15791) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - CHP1 Rabbit pAb (A15791)

Immunofluorescence analysis of L929 cells using CHP1 Rabbit pAb (A15791) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CHP1 Rabbit pAb (A15791)

Immunofluorescence analysis of U-2 OS cells using CHP1 Rabbit pAb (A15791) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCHP1 Rabbit pAb
Catalog No.A15791
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human CHP1 (NP_009167.1).
SequenceMGSRASTLLRDEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAFFPEGEDQVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKISRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSAISFTEFVKVLEKVDVEQKMSIRFLH
Gene ID
Swiss Prot
SynonymsCHP; p22; p24; SPAX9; Sid470p; SLC9A1BP; CHP1
Calculated MW22kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, 293T, HeLa, Mouse brain, Mouse heart, Mouse kidney, Rat brain
Cellular locationCell membrane, Cytoplasm, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Lipid-anchor, Membrane, Nucleus, cytoskeleton

Documents

Certificate of Compliance

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    ABclonal:Western blot - CHP1 Rabbit pAb (A15791)}

    Western blot - CHP1 Rabbit pAb (A15791)

    Western blot analysis of various lysates using CHP1 Rabbit pAb (A15791) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CHP1 Rabbit pAb (A15791)}

    Immunofluorescence - CHP1 Rabbit pAb (A15791)

    Immunofluorescence analysis of L929 cells using CHP1 Rabbit pAb (A15791) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - CHP1 Rabbit pAb (A15791)}

    Immunofluorescence - CHP1 Rabbit pAb (A15791)

    Immunofluorescence analysis of U-2 OS cells using CHP1 Rabbit pAb (A15791) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.