제품 > 항체 > 다중클론항체(pAb)

CHRNA5 Rabbit pAb (A1741)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CHRNA5 Rabbit pAb (A1741)

Western blot analysis of extracts of various cell lines, using CHRNA5 antibody (A1741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameCHRNA5 Rabbit pAb
Catalog No.A1741
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-240 of human CHRNA5 (NP_000736.2).
SequenceRCGLAGAAGGAQRGLSEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTPDIVLFDNADGRFEGTSTKTVIRYNGTVTWTPPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDKRDFFDNGEWEIVSATGSKGNRTDSCCWYPYV
Gene ID
Swiss Prot
SynonymsLNCR2
Calculated MW53kDa
Observed MW50kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, U-251MG, Jurkat, MCF-7, Mouse brain, Mouse stomach
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse

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ABclonal:Western blot - CHRNA5 Rabbit pAb (A1741)}

Western blot - CHRNA5 Rabbit pAb (A1741)

Western blot analysis of extracts of various cell lines, using CHRNA5 antibody (A1741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.