제품 > 항체 > 다중클론항체(pAb)

CHST2 Rabbit pAb (A15755)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CHST2 Rabbit pAb (A15755)

Western blot analysis of lysates from U-251MG cells, using CHST2 Rabbit pAb (A15755) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameCHST2 Rabbit pAb
Catalog No.A15755
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 231-530 of human CHST2 (NP_004258.2).
SequenceFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVDDRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLRDPALDLKVIHLVRDPRAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGVGGPADYHALGAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL
Gene ID
Swiss Prot
SynonymsC6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1; CHST2
Calculated MW58kDa
Observed MW60kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-251MG
Cellular locationGolgi apparatus, Single-pass type II membrane protein, trans-Golgi network membrane
    ABclonal:Western blot - CHST2 Rabbit pAb (A15755)}

    Western blot - CHST2 Rabbit pAb (A15755)

    Western blot analysis of lysates from U-251MG cells, using CHST2 Rabbit pAb (A15755) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.