제품 > 항체 > 다중클론항체(pAb)

CHTF18 Rabbit pAb (A17752)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - CHTF18 Rabbit pAb (A17752)

Western blot analysis of lysates from HeLa cells, using CHTF18 Rabbit pAb (A17752) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CHTF18 Rabbit pAb (A17752)

Immunofluorescence analysis of C6 cells using CHTF18 Rabbit pAb (A17752) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCHTF18 Rabbit pAb
Catalog No.A17752
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a component of a replication factor C (RFC) complex, which loads proliferating cell nuclear antigen (PCNA) on to DNA during the S phase of cell cycle. The encoded protein may interact with other proteins, including RFC complex 3, to form a clamp loader complex that plays a role in sister chromatid cohesion during metaphase-anaphase transition.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 794-975 of human CHTF18 (NP_071375.1).
SequenceGTMLAYSLTYRQERTPDGQYIYRLEPNVEELCRFPELPARKPLTYQTKQLIAREIEVEKMRRAEASARVENSPQVDGSPPGLEGLLGGIGEKGVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL
Gene ID
Swiss Prot
SynonymsCHL12; Ctf18; RUVBL; C16orf41; C321D2.2; C321D2.3; C321D2.4; CHTF18
Calculated MW107kDa
Observed MW130kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationcytosol, nucleoplasm, nucleus

Documents

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    ABclonal:Western blot - CHTF18 Rabbit pAb (A17752)}

    Western blot - CHTF18 Rabbit pAb (A17752)

    Western blot analysis of lysates from HeLa cells, using CHTF18 Rabbit pAb (A17752) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - CHTF18 Rabbit pAb (A17752)}

    Immunofluorescence - CHTF18 Rabbit pAb (A17752)

    Immunofluorescence analysis of C6 cells using CHTF18 Rabbit pAb (A17752) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.