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CLCC1 Rabbit pAb (A17656)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CLCC1 Rabbit pAb (A17656)

Western blot analysis of various lysates using CLCC1 Rabbit pAb (A17656) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - CLCC1 Rabbit pAb (A17656)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CLCC1 Rabbit pAb (A17656) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameCLCC1 Rabbit pAb
Catalog No.A17656
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable chloride channel activity. Predicted to be involved in chloride transport. Located in endoplasmic reticulum and mitochondria-associated endoplasmic reticulum membrane. Implicated in retinitis pigmentosa 32.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 302-501 of human CLCC1 (NP_055942.1).
SequenceGAGKSVHVLRHIGGPESEPPQALRPRDRRRQEEIDYRPDGGAGDADFHYRGQMGPTEQGPYAKTYEGRREILRERDVDLRFQTGNKSPEVLRAFDVPDAEAREHPTVVPSHKSPVLDTKPKETGGILGEGTPKESSTESSQSAKPVSGQDTSGNTEGSPAAEKAQLKSEAAGSPDQGSTYSPARGVAGPRGQDPVSSPCG
Gene ID
Swiss Prot
SynonymsMCLC; RP32; CLCC1
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, 22Rv1, Mouse spleen
Cellular locationendoplasmic reticulum, nuclear membrane

Documents

Certificate of Compliance

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    ABclonal:Western blot - CLCC1 Rabbit pAb (A17656)}

    Western blot - CLCC1 Rabbit pAb (A17656)

    Western blot analysis of various lysates using CLCC1 Rabbit pAb (A17656) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunohistochemistry - CLCC1 Rabbit pAb (A17656)}

    Immunohistochemistry - CLCC1 Rabbit pAb (A17656)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CLCC1 Rabbit pAb (A17656) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.