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CLCN1 Rabbit pAb (A5739)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CLCN1 Rabbit pAb (A5739)

Western blot analysis of lysates from Mouse skeletal muscle, using CLCN1 Rabbit pAb (A5739) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CLCN1 Rabbit pAb (A5739)

Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using CLCN1 Rabbit pAb (A5739) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CLCN1 Rabbit pAb (A5739)

Immunohistochemistry analysis of paraffin-embedded Rat skeletal muscle using CLCN1 Rabbit pAb (A5739) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCLCN1 Rabbit pAb
Catalog No.A5739
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 779-988 of human CLCN1 (NP_000074.3).
SequenceARPTKKKTTQDSTDLVDNMSPEEIEAWEQEQLSQPVCFDSCCIDQSPFQLVEQTTLHKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSTRKSTGAPPSSAENWNLPEDRPGATGTGDVIAASPETPVPSPSPEPPLSLAPGKVEGELEELELVESPGLEEELADILQGPSLRSTDEEDEDELIL
Gene ID
Swiss Prot
SynonymsCLC1; CLCN1
Calculated MW109kDa
Observed MW109kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse skeletal muscle
Cellular locationMembrane, Multi-pass membrane protein

Documents

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    ABclonal:Western blot - CLCN1 Rabbit pAb (A5739)}

    Western blot - CLCN1 Rabbit pAb (A5739)

    Western blot analysis of lysates from Mouse skeletal muscle, using CLCN1 Rabbit pAb (A5739) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - CLCN1 Rabbit pAb (A5739)}

    Immunohistochemistry - CLCN1 Rabbit pAb (A5739)

    Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using CLCN1 Rabbit pAb (A5739) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - CLCN1 Rabbit pAb (A5739)}

    Immunohistochemistry - CLCN1 Rabbit pAb (A5739)

    Immunohistochemistry analysis of paraffin-embedded Rat skeletal muscle using CLCN1 Rabbit pAb (A5739) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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