제품 > 항체 > 다중클론항체(pAb)

CLC Rabbit pAb (A15265)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CLC Rabbit pAb (A15265)

Western blot analysis of various lysates using CLC Rabbit pAb (A15265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90S.

Overview

Product nameCLC Rabbit pAb
Catalog No.A15265
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CLC (NP_001819.2).
SequenceMSLLPVPYTEAASLSTGSTVTIKGRPLACFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREYGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR
Gene ID
Swiss Prot
SynonymsGAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN; CLC
Calculated MW16kDa
Observed MW14kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, HL-60
Cellular locationCytoplasm, Cytoplasmic granule, cytosol
    ABclonal:Western blot - CLC Rabbit pAb (A15265)}

    Western blot - CLC Rabbit pAb (A15265)

    Western blot analysis of various lysates using CLC Rabbit pAb (A15265) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90S.

    * For research use only. Not for therapeutic or diagnostic purposes.