제품 > 항체 > 다중클론항체(pAb)

CMIP Rabbit pAb (A20487)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CMIP Rabbit pAb (A20487)

Western blot analysis of various lysates using CMIP Rabbit pAb (A20487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - CMIP Rabbit pAb (A20487)

Western blot analysis of lysates from K-562 cells, using CMIP Rabbit pAb (A20487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CMIP Rabbit pAb (A20487)

Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using CMIP Rabbit pAb (A20487) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameCMIP Rabbit pAb
Catalog No.A20487
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a c-Maf inducing protein that plays a role in T-cell signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 640-773 of human CMIP (NP_938204.2).
SequenceRLTLPSKSTDADLARLLSSGSFGNLENLSLAFTNVTSACAEHLIKLPSLKQLNLWSTQFGDAGLRLLSEHLTMLQVLNLCETPVTDAGLLALSSMKSLCSLNMNSTKLSADTYEDLKAKLPNLKEVDVRYTEAW
Gene ID
Swiss Prot
SynonymsTCMIP; CMIP
Calculated MW86kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, Mouse testis, Mouse kidney, Rat kidney
Cellular locationcytosol, nucleoplasm

Documents

Certificate of Compliance

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    ABclonal:Western blot - CMIP Rabbit pAb (A20487)}

    Western blot - CMIP Rabbit pAb (A20487)

    Western blot analysis of various lysates using CMIP Rabbit pAb (A20487) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - CMIP Rabbit pAb (A20487)}

    Western blot - CMIP Rabbit pAb (A20487)

    Western blot analysis of lysates from K-562 cells, using CMIP Rabbit pAb (A20487) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - CMIP Rabbit pAb (A20487)}

    Immunohistochemistry - CMIP Rabbit pAb (A20487)

    Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using CMIP Rabbit pAb (A20487) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.