제품 > 항체 > 다중클론항체(pAb)

CNNM1 Rabbit pAb (A17131)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CNNM1 Rabbit pAb (A17131)

Western blot analysis of various lysates using CNNM1 Rabbit pAb (A17131) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of C6 cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of L929 cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of U-2 OS cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCNNM1 Rabbit pAb
Catalog No.A17131
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the ancient conserved domain protein family. The encoded protein may bind copper. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 692-951 of human CNNM1 (NP_065081.2).
SequenceNGAFTYYGVPAIMTTACSDNDVRKVGSLAGSSVFLNRSPSRCSGLNRSESPNRERSDFGGSNTQLYSSSNNLYMPDYSVHILSDVQFVKITRQQYQNALTACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAITLLNNRNSLPCSRSDGLRSPSEVVYLRMEELAFTQEEMTDFEEHSTQQLTLSPAAVPTRAASDSECCNINLDTETSPCSSDFEENVGKKLLRTLSGQKRKRSPEGERTSEDNSNLTPLIT
Gene ID
Swiss Prot
SynonymsACDP1; CLP-1; CNNM1
Calculated MW104kDa
Observed MW104kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationplasma membrane

Documents

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ABclonal:Western blot - CNNM1 Rabbit pAb (A17131)}

Western blot - CNNM1 Rabbit pAb (A17131)

Western blot analysis of various lysates using CNNM1 Rabbit pAb (A17131) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)}

Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of C6 cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)}

Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of L929 cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CNNM1 Rabbit pAb (A17131)}

Immunofluorescence - CNNM1 Rabbit pAb (A17131)

Immunofluorescence analysis of U-2 OS cells using CNNM1 Rabbit pAb (A17131) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.