제품 > 항체 > 다중클론항체(pAb)

CNPY2 Rabbit pAb (A12197)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CNPY2 Rabbit pAb (A12197)

Western blot analysis of various lysates using CNPY2 Rabbit pAb (A12197) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - CNPY2 Rabbit pAb (A12197)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CNPY2 Rabbit pAb (A12197) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCNPY2 Rabbit pAb
Catalog No.A12197
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be active in endoplasmic reticulum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CNPY2 (NP_055070.1).
SequenceMKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL
Gene ID
Swiss Prot
SynonymsMSAP; TMEM4; ZSIG9; HP10390; CNPY2
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesBxPC-3, SGC-7901, HeLa, HepG2, Mouse liver, Mouse kidney
Cellular locationEndoplasmic reticulum.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - CNPY2 Rabbit pAb (A12197)}

    Western blot - CNPY2 Rabbit pAb (A12197)

    Western blot analysis of various lysates using CNPY2 Rabbit pAb (A12197) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - CNPY2 Rabbit pAb (A12197)}

    Immunohistochemistry - CNPY2 Rabbit pAb (A12197)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CNPY2 Rabbit pAb (A12197) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.