제품 > 항체 > 다중클론항체(pAb)

COG1 Rabbit pAb (A17594)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COG1 Rabbit pAb (A17594)

Western blot analysis of lysates from 22Rv1 cells, using COG1 Rabbit pAb (A17594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)

Immunohistochemistry analysis of paraffin-embedded Human colon using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameCOG1 Rabbit pAb
Catalog No.A17594
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is one of eight proteins (Cog1-8) which form a Golgi-localized complex (COG) required for normal Golgi morphology and function. It is thought that this protein is required for steps in the normal medial and trans Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 835-935 of human COG1 (NP_061184.1).
SequenceDSRIEKVTDHLEALIDPFDLDVFTPHLNSNLHRLVQRTSVLFGLVTGTENQLAPRSSTFNSQEPHNILPLASSQIRFGLLPLSMTSTRKAKSTRNIETKAQ
Gene ID
Swiss Prot
SynonymsLDLB; CDG2G; COG1
Calculated MW109kDa
Observed MW109kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples22Rv1
Cellular locationGolgi apparatus, Golgi transport complex

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - COG1 Rabbit pAb (A17594)}

    Western blot - COG1 Rabbit pAb (A17594)

    Western blot analysis of lysates from 22Rv1 cells, using COG1 Rabbit pAb (A17594) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)}

    Immunohistochemistry - COG1 Rabbit pAb (A17594)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)}

    Immunohistochemistry - COG1 Rabbit pAb (A17594)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - COG1 Rabbit pAb (A17594)}

    Immunohistochemistry - COG1 Rabbit pAb (A17594)

    Immunohistochemistry analysis of paraffin-embedded Human colon using COG1 Rabbit pAb (A17594) at dilution of 1:50 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.