제품 > 항체 > 다중클론항체(pAb)

COL16A1 Rabbit pAb (A18837)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COL16A1 Rabbit pAb (A18837)

Western blot analysis of lysates from PC-3 cells, using COL16A1 Rabbit pAb (A18837) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - COL16A1 Rabbit pAb (A18837)

Western blot analysis of various lysates using COL16A1 Rabbit pAb (A18837) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameCOL16A1 Rabbit pAb
Catalog No.A18837
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the alpha chain of type XVI collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Members of this collagen family are found in association with fibril-forming collagens such as type I and II, and serve to maintain the integrity of the extracellular matrix. High levels of type XVI collagen have been found in fibroblasts and keratinocytes, and in smooth muscle and amnion.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human COL16A1 (NP_001847.3).
SequencePRAEEARGDNSEGDPGCVGSPGLPGPPGLPGQRGEEGPPGMRGSPGPPGPIGPPGFPGAVGSPGLPGLQGERGLTGLTGDKGEPGPPGQPGYPGATGPPGL
Gene ID
Swiss Prot
Synonyms447AA; FP1572; COL16A1
Calculated MW158kDa
Observed MW145kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3, Mouse kidney, Mouse heart
Cellular locationendoplasmic reticulum lumen, extracellular region, extracellular space

Documents

Certificate of Compliance

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    ABclonal:Western blot - COL16A1 Rabbit pAb (A18837)}

    Western blot - COL16A1 Rabbit pAb (A18837)

    Western blot analysis of lysates from PC-3 cells, using COL16A1 Rabbit pAb (A18837) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - COL16A1 Rabbit pAb (A18837)}

    Western blot - COL16A1 Rabbit pAb (A18837)

    Western blot analysis of various lysates using COL16A1 Rabbit pAb (A18837) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.