제품 > 항체 > 다중클론항체(pAb)

COL4A3BP Rabbit pAb (A15376)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COL4A3BP Rabbit pAb (A15376)

Western blot analysis of various lysates using COL4A3BP Rabbit pAb (A15376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - COL4A3BP Rabbit pAb (A15376)

Immunofluorescence analysis of L929 cells using COL4A3BP Rabbit pAb (A15376) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCOL4A3BP Rabbit pAb
Catalog No.A15376
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 369-598 of human COL4A3BP (NP_112729.1).
SequenceQKVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF
Gene ID
Swiss Prot
SynonymsCERT; GPBP; CERTL; MRD34; STARD11; COL4A3BP
Calculated MW71kDa
Observed MW71kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, A-549, HeLa, Mouse thymus, Mouse brain, Mouse heart
Cellular locationCytoplasm, Endoplasmic reticulum, Golgi apparatus

Documents

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ABclonal:Western blot - COL4A3BP Rabbit pAb (A15376)}

Western blot - COL4A3BP Rabbit pAb (A15376)

Western blot analysis of various lysates using COL4A3BP Rabbit pAb (A15376) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - COL4A3BP Rabbit pAb (A15376)}

Immunofluorescence - COL4A3BP Rabbit pAb (A15376)

Immunofluorescence analysis of L929 cells using COL4A3BP Rabbit pAb (A15376) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.