제품 > 항체 > 다중클론항체(pAb)

COLGALT2 Rabbit pAb (A15407)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - COLGALT2 Rabbit pAb (A15407)

Western blot analysis of various lysates using COLGALT2 Rabbit pAb (A15407) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCOLGALT2 Rabbit pAb
Catalog No.A15407
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable procollagen galactosyltransferase activity. Predicted to be involved in collagen fibril organization. Predicted to be located in endoplasmic reticulum lumen.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 28-230 of human COLGALT2 (NP_055916.1).
SequenceRFVAERDSEDDGEEPVVFPESPLQSPTVLVAVLARNAAHTLPHFLGCLERLDYPKSRMAIWAATDHNVDNTTEIFREWLKNVQRLYHYVEWRPMDEPESYPDEIGPKHWPTSRFAHVMKLRQAALRTAREKWSDYILFIDVDNFLTNPQTLNLLIAENKTIVAPMLESRGLYSNFWCGITPKGFYKRTPDYVQIREWKRTGCF
Gene ID
Swiss Prot
SynonymsC1orf17; GLT25D2; ColGalT 2; COLGALT2
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLO2, Mouse brain
Cellular locationEndoplasmic reticulum lumen
    ABclonal:Western blot - COLGALT2 Rabbit pAb (A15407)}

    Western blot - COLGALT2 Rabbit pAb (A15407)

    Western blot analysis of various lysates using COLGALT2 Rabbit pAb (A15407) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.