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COPS2 Rabbit pAb (A7037)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COPS2 Rabbit pAb (A7037)

Western blot analysis of various lysates using COPS2 Rabbit pAb (A7037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - COPS2 Rabbit pAb (A7037)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using COPS2 Rabbit pAb (A7037) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - COPS2 Rabbit pAb (A7037)

Immunofluorescence analysis of A-549 cells using COPS2 Rabbit pAb (A7037).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Overview

Product nameCOPS2 Rabbit pAb
Catalog No.A7037
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable transcription corepressor activity. Involved in protein deneddylation and protein phosphorylation. Located in cytoplasm. Part of COP9 signalosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human COPS2 (NP_004227.1).
SequenceMSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFKTNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILA
Gene ID
Swiss Prot
SynonymsCSN2; SGN2; ALIEN; TRIP15; COPS2
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, HeLa, A-431, Jurkat, NIH/3T3, Mouse brain, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - COPS2 Rabbit pAb (A7037)}

Western blot - COPS2 Rabbit pAb (A7037)

Western blot analysis of various lysates using COPS2 Rabbit pAb (A7037) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - COPS2 Rabbit pAb (A7037)}

Immunohistochemistry - COPS2 Rabbit pAb (A7037)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using COPS2 Rabbit pAb (A7037) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - COPS2 Rabbit pAb (A7037)}

Immunofluorescence - COPS2 Rabbit pAb (A7037)

Immunofluorescence analysis of A-549 cells using COPS2 Rabbit pAb (A7037).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.